Thermo Fisher Scientific IFN gamma Polyclonal Antibody
Rhesus macaque IFN-gamma를 검출하는 폴리클로날 항체로, WB, ELISA, Neutralization에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 교차 반응성이 낮고 보존성이 우수합니다. 연구용으로만 사용 가능합니다.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IFN gamma Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| ELISA (Detection) | 0.1–0.4 µg/mL |
| ELISA (Capture) | 0.2–0.8 µg/mL |
| Neutralization (Neu) | 1.5–5.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rhesus monkey |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant rhesus macaque IFN-gamma (Gln24–Gln165) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2610701 |
Product Specific Information
This antibody detects rhesus macaque IFN-gamma in ELISAs and Western blots.
In sandwich ELISAs, less than 30% cross-reactivity with recombinant human IFN-gamma is observed, and less than 0.1% cross-reactivity with recombinant mouse, rat, porcine, canine, cotton rat, and feline IFN-gamma is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endotoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, and cytokines.
It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits. On SDS-PAGE, it appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
The biological activity of IFN gamma is highly species-specific; human IFN gamma does not cross-react with mouse IFN gamma.
Functions include:
- Antiviral and antiproliferative activity
- Induction of class I and II MHC
- Macrophage activation
- Enhancement of immunoglobulin secretion by B lymphocytes
IFN gamma acts through the JAK-STAT pathway via receptor I and II binding and is regulated by cytokines such as IL2, FGF basic, and EGF. It is downregulated by vitamin D3 or DMN.
Mutations in the IFN gamma gene are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.