Thermo Fisher Scientific VAMP 2,1 Polyclonal Antibody
Rabbit polyclonal antibody against VAMP2/VAMP1, reactive with human, mouse, and rat. Suitable for WB and IHC at 10–50 µg/mL. Lyophilized form, reconstitute in sterile water. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 10–50 µg/mL | - |
| Immunohistochemistry (IHC) | 10–50 µg/mL | - |
| Miscellaneous (PubMed) | - | View 2 publications |
Product Specifications
| Category | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from amino acid region 10–60 of human VAMP2 conjugated to a blue carrier protein. The VAMP2 peptide shares 93% identity with VAMP1. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4 °C for short term. For long-term storage, store at −20 °C avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
- The peptide is homologous in rat, mouse, and xenopus.
- Specificity: Recognizes both VAMP2 and VAMP1.
Target Information
Vesicle-associated membrane protein 2 (VAMP2) is essential for cAMP-regulated exocytosis. Also known as synaptobrevin, it is part of the synaptic vesicle docking and fusion complex and plays a central role in neuroexocytosis.
Two isoforms, VAMP1 and VAMP2, are expressed in the nervous system and various tissues including kidney, adrenal gland, liver, pancreas, thyroid, heart, and smooth muscle.
VAMP1 is mainly found in exocrine pancreas and kidney tubular cells, while VAMP2 predominates in Langerhans islets and glomerular cells. Both show vesicular intracellular distribution.
Neuronal VAMP proteins are important for cellular physiology across tissues.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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