Thermo Fisher Scientific VAMP 2,1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

10-50 µg/mL

-

Immunohistochemistry (IHC)

10-50 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from aa region 10-60 of human VAMP2 conjugated to blue carrier protein was used as the antigen. The VAMP2 peptide shares 93% shared identity with VAMP1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:VAMP1/VAMP2,uniProtId:P63027-1,ncbiNodeId:9606,antigenRange:10-60,antigenLength:116,antigenImageFileName:OSS00045G-500UG_VAMP1VAMP2_P63027-1_Rabbit.svg,antigenImageFileNamePDP:OSS00045G-500UG_VAMP1VAMP2_P63027-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

The peptide is homologous in many other species including rat, mouse and xenopus.

Specificity of this antibody: VAMP2 and VAMP1.

Target Information

Vesicle-associated membrane protein 2 is essential for cAMP-regulated exocytosis. VAMP (also know as synaptobrevin) is part of the synaptic vesicle docking and fusion complex and plays a central role in neuroexocytosis. Two VAMP (vesicle-associated membrane protein) isoforms are expressed in the nervous system and are differently distributed among the specialized parts of the tissue. VAMP-1 and -2 are present in all tissues tested, including kidney, adrenal gland, liver, pancreas, thyroid, heart, and smooth muscle. The two isoforms are differentially expressed in various tissues and their level may depend on differentiation. VAMP-1 is restricted to exocrine pancreas and to kidney tubular cells, whereas VAMP-2 is the predominant isoform present in Langerhans islets and in glomerular cells. Both isoforms show a patchy vesicular intracellular distribution in confocal microscopy. Evidence for the importance of neuronal VAMPproteins in the physiology of all cells is indicated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.