Thermo Fisher Scientific CD5 Monoclonal Antibody (CD5, 2416)
인간 CD5 단백질을 인식하는 Mouse 모노클로날 항체로 다양한 면역분석(WB, IHC, ICC, Flow)에 사용 가능. 비결합형 액상 형태로 200 µg/mL 농도. 단백질 A/G로 정제되었으며 PBS 버퍼에 보존. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/10^6 cells |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CD5, 2416 |
| Immunogen | Recombinant fragment of human CD5 protein (around aa 269–366) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein.
It is present on all mature T-lymphocytes, most thymocytes, and many T-cell leukemias and lymphomas.
CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor regulating T-cell proliferation.
CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes.
In lymph nodes, the main reactivity is observed in T-cell areas.
Diseases associated with CD5 dysfunction include thymus cancer and Richter’s Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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