Thermo Fisher Scientific Carbonic Anhydrase IX Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

-

ELISA (ELISA)

1:5,000-1:10,000

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Mouse Carbonic Anhydrase IX/CA9 Protein (Met1-Asp390) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Carbonic Anhydrase IX,uniProtId:Q8VHB5-1,ncbiNodeId:10090,antigenRange:1-390,antigenLength:437,antigenImageFileName:PA5-81331_Carbonic_Anhydrase_IX_Q8VHB5-1_Rabbit.svg,antigenImageFileNamePDP:PA5-81331_Carbonic_Anhydrase_IX_Q8VHB5-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7

Contains

0.03% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2788547

Product Specific Information

This antibody has specificity for Mouse Carbonic Anhydrase IX/CA9.

Target Information

Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.