Thermo Fisher Scientific Rabbit anti-Mouse IgG (H+L) Secondary Antibody, Biotin
Rabbit anti-Mouse IgG (H+L) Biotin 결합 2차 항체로, WB, IHC, ELISA 등 다양한 면역검출 실험에 사용 가능. 고순도 IgG 분획 정제, 높은 민감도와 신호 증폭 효과 제공. 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications 및 권장 희석 배수
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:300,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Mouse IgG whole molecule |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl, 1% BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10986909 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting, or purification of their specified target.
Secondary antibodies offer increased versatility, enabling users to use many detection systems (e.g., HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
Additional Formats
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- AP
- FITC
- HRP
- Rhodamine
- TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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