Thermo Fisher Scientific Hin1I (BsaHI) (10 U/μL)

Thermo Fisher Scientific Hin1I (BsaHI) (10 U/μL)

Hin1I (BsaHI)는 GR^CGYC 염기서열을 인식하는 제한효소로, G 버퍼에서 37°C에서 최적의 절단 활성을 나타냅니다.

인식 서열

5' G R ↓C G Y C 3'
3' C Y G C ↑R G 5'

Hin1I (BsaHI) restriction enzyme recognizes GR^CGYC sites and cuts best at 37°C in G buffer.
See Reaction Conditions for Restriction Enzymes for detailed activity and double digestion conditions.
Note: Also available as a FastDigest enzyme for rapid DNA digestion.
Isoschizomers: AcyI, BsaHI, BssNI, BstACI, Hsp92I

제품 특징

• Superior quality — stringent quality control and industry-leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double digestions
• BSA premixed in reaction buffers for enhanced stability
• Wide selection of restriction endonuclease specificities

응용 분야

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

주의 사항

Hin1I 절단은 dcm 메틸화가 중첩될 경우 저해됩니다.
이를 방지하기 위해 dam-, dcm- strain (예: GM2163)을 사용하십시오.
메틸화 민감도는 제품 사양을 참조하십시오.

제품 사양

제품 이미지

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