Thermo Fisher Scientific Citrulline Monoclonal Antibody (6C2.1), APC
Citrulline 잔기 특이적 단일클론 항체로 다양한 응용(WB, IHC, ICC, ELISA)에 사용 가능. APC 형광 표지로 651/660 nm에서 검출. 단백질 G 정제, 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6C2.1 |
| Immunogen | Synthetic L-Citrulline conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932211 |
Additional Formats
- Unconjugated: MA5-27574
- FITC: MA5-45758
- PE: MA5-45760
- PerCP: MA5-45759
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45757 was sufficient for detection of Citrulline in 2 µg of Citrulline conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for citrulline residues on modified proteins. Tested using citrulline conjugated Bovine Serum Albumin (BSA).
Target Information
Citrulline is an amino acid that is not incorporated into proteins during synthesis but is generated within proteins by the deimination of arginine. Citrulline occurs normally in many proteins, such as Myelin Basic Protein (MBP), while others, such as fibrin, become citrullinated during inflammation or apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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