Thermo Scientific™

ActivX™ TAMRA-FP Serine Hydrolase Probe

The TAMRA-FP probe feature a reactive fluorophosphonate group that is used to label and detect active serine hydrolases by fluorescent자세히 알아보기

The TAMRA-FP probe feature a reactive fluorophosphonate group that is used to label and detect active serine hydrolases by fluorescent gel imaging, capillary electrophoresis or mass spectrometry. An anti-TAMRA antibody is also available for immuno-enrichment of TAMRA-FP probe-labeled proteins.

ActivX™ active-site enrichment probes are advantageous for determining active enzyme levels through the use of analog-tagged probes to allow profiling of both inactive and active enzymes. The ActivX probes selectively enrich only those enzymes that are functionally active and biologically relevant at the time of labeling. With selective enrichment, one can identify and profile target enzyme classes across samples or can assess the specificity and affinity of enzyme inhibitors.

The serine hydrolase superfamily is one of the largest, most diverse enzyme families in eukaryotic proteomes. Serine hydrolases are generally grouped into two large families: serine proteases (e.g., trypsin, elastase and thrombin) and metabolic serine hydrolases. Metabolic serine hydrolases are divided into multiple enzyme subclasses (e.g., esterases, lipases, amidases and peptidases) based on structure, catalytic mechanism and substrate preference. These probes can be used to assess activity or screen small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins.

These products are subject to a limited use label license.

사양

설명ActivX™ TAMRA-FP Serine Hydrolase Probe

제제TAMRA-fluorophosphonate, MW 680

어세이Protein Quantitation Assay

검출 방법Chemiluminescence, Mass Spectrometry, Western Blot

기질 유형Serine Hydrolase Substrate

제품라인ActivX™

수량6.8 μg

용도(애플리케이션)Pull-Down

타겟Serine Hydrolase

유형Fluorophosphonate (FP) probe

라벨 또는 염료Other Label(s) or Dye(s)

형식Tube, Spin Column

Unit SizeEach