Thermo Fisher Scientific RBM25 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human RBM25. Recombinant protein control fragment (Product #RP-89085). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RBM25,uniProtId:P49756-1,ncbiNodeId:9606,antigenRange:690-821,antigenLength:843,antigenImageFileName:PA5-51798_RBM25_P49756-1_Rabbit.svg,antigenImageFileNamePDP:PA5-51798_RBM25_P49756-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2646376

Product Specific Information

Immunogen sequence: PVDSVFNKFE DEDSDDVPRK RKLVPLDYGE DDKNATKGTV NTEEKRKHIK SLIEKIPTAK PELFAYPLDW SIVDSILMER RIRPWINKKI IEYIGEEEAT LVDFVCSKVM AHSSPQSILD DVAMVLDEEA EV

Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 99%.

Target Information

RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5-splice site (5-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5-ss usage through the 5-CGGGCA-3RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5 ss in a 5-CGGGCA-3-dependent manner. Binds to the exonic splicing enhancer 5-CGGGCA-3 RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.