Invitrogen™

Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit

The Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit is designed for rapid cloning with a Gateway™ entry clone and subsequent자세히 알아보기

The Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit is designed for rapid cloning with a Gateway™ entry clone and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. In addition to the T7 promoter, each vector contains only the necessary functional elements and an N- or C-terminal 6xHis tag (pET300/NT-DEST and pET301/CT-DEST, respectively) for convenient purification and detection (Figure 1). The vector kit is ideal for structural biologists who desire no or minimal modifications to their protein of interest. To maximize expression, use with MagicMedia™ E. coli Expression Medium.

*Contents and Storage:
*The Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit includes 6 μg each of pET300/NT-DEST and pET301/CT-DEST vectors and 10 μg of control vector. Store at -20“C. Guaranteed stable for 6 months when properly stored.

사양

구성 또는 유도성 시스템Inducible

유도제IPTG

프로모터T7

제품 유형DNA Cloning Vector Kit

선택 제제(진핵)None

항생제 내성 박테리아Ampicillin (AmpR)

단백질 태그His Tag (6x)

클로닝 방법Gateway

수량6 μg

벡터pET, pDEST

제품라인Champion™, Gateway™

Unit SizeEach