Thermo Fisher Scientific Champion pET300/NT-DEST and pET301/CT-DEST Gateway Vector Kit

Invitrogen™ Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit

The Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit is designed for rapid cloning using a Gateway™ entry clone and for subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter.
Each vector contains only the necessary functional elements and an N- or C-terminal 6xHis tag (pET300/NT-DEST and pET301/CT-DEST, respectively) for convenient purification and detection.

This vector kit is ideal for structural biologists who require no or minimal modifications to their protein of interest.
To maximize expression, use with MagicMedia™ E. coli Expression Medium.

Contents and Storage

The Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit includes:

• 6 μg each of pET300/NT-DEST and pET301/CT-DEST vectors
• 10 μg of control vector

Storage: Store at -20°C. Guaranteed stable for 6 months when properly stored.

Specifications

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