
Thermo Fisher Scientific DDDDK Polyclonal Antibody
DDDDK(FLAG) 태그 인식용 Thermo Fisher Scientific의 폴리클로날 항체로, WB·ELISA·IP 등 다양한 단백질 분석에 적합. Rabbit IgG 기반으로 높은 특이성과 안정성 제공. 연구용 단백질 검출 및 정제에 최적화된 항체.
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Thermo Fisher Scientific DDDDK Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to DDDDK tag (Sequence: DYKDDDDK) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence commonly used as a protein tag to facilitate protein purification, detection, and localization.
The tag consists of eight amino acids: Asp–Tyr–Lys–Asp–Asp–Asp–Asp–Lys.
It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation using anti-FLAG antibodies.
The DYKDDDDK tag is widely used in molecular biology and biochemistry research, including protein–protein interaction studies, protein expression analysis, and protein localization studies.
The FLAG tag structure has been optimized for compatibility with attached proteins, being more hydrophilic than other epitope tags and less likely to denature or inactivate target proteins.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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