Thermo Fisher Scientific GeneArt CRISPR Nuclease Vector with OFP Reporter Kit

Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit

The GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter.
The OFP reporter allows fluorescence-based tracking of transfection efficiency and FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells.

The linearized GeneArt™ CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease.
A version with competent cells is also available (Cat. No. A21178).

주요 특징

• Easy-to-design genome engineering system
• Affordable, ready-to-use cloning vectors
• Enrichment for hard to transfect or difficult to modify cell lines

All-in-one Vector System for CRISPR-based Genome Editing

The GeneArt™ CRISPR Nuclease Vector kit offers a simple, ready-to-use, all-in-one expression vector system consisting of both a Cas9 nuclease expression cassette and a guide RNA (gRNA) cloning cassette for rapid and efficient cloning of DNA that encodes target-specific CRISPR RNA (crRNA).
This system enables sequence-specific genome editing from a single plasmid.

After relevant targets have been identified using GeneArt™ CRISPR vectors, biologically relevant mutations can be precisely created with GeneArt™ Precision TALs, offering high specificity and low off-target effects.

CRISPR gRNA 디자인 지원

Our CRISPR Search & Design tool allows you to:

• Search a database of over 600,000 predesigned CRISPR gRNAs in human and mouse genes
• Analyze custom sequences for de novo gRNA designs using proprietary algorithms
• Optimize sequences for gene knockout targeting the first three transcribed exons

Search results include:

• Top 6 CRISPR sequences with PAM sites
• Exon maps with gRNA binding sites
• Potential off-target binding sites for each CRISPR sequence

The tool designs the correct gRNA format for your GeneArt™ CRISPR Nuclease Vector.
Start designing today >

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