Thermo Fisher Scientific SIRT1 Monoclonal Antibody (1F3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1F3

Immunogen

Purified recombinant fragment of human SIRT1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10978176

Product Specific Information

MA5-15677 targets SIRT1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human and Non-human primate samples.

The MA5-15677 immunogen is purified recombinant fragment of human SIRT1 expressed in E. Coli.

MA5-15677 detects SIRT1 which has a predicted molecular weight of approximately 120kDa.

Target Information

NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, repsonse to DNA damage, metabolism, apoptosis, and autophagy. SIRT1 can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histone and DNA, leading to transcriptional repression. It is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5`-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.