Thermo Fisher Scientific SIRT1 Monoclonal Antibody (1F3)
SIRT1 단백질을 검출하기 위한 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 1F3). 다양한 응용 분야(WB, IHC, ICC/IF, Flow, ELISA)에서 사용 가능. Human 및 Non-human primate 시료 반응성. 고순도 재조합 SIRT1 항원을 이용한 높은 특이성 제공.
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Thermo Fisher Scientific SIRT1 Monoclonal Antibody (1F3)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | - |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F3 |
| Immunogen | Purified recombinant fragment of human SIRT1 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10978176 |
Product Specific Information
MA5-15677 targets SIRT1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human and Non-human primate samples.
The immunogen is purified recombinant fragment of human SIRT1 expressed in E. coli.
MA5-15677 detects SIRT1 with a predicted molecular weight of approximately 120 kDa.
Target Information
NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of various cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis, and autophagy.
SIRT1 modulates chromatin function through deacetylation of histones and promotes alterations in histone and DNA methylation, leading to transcriptional repression.
It is essential in skeletal muscle cell differentiation and mediates the inhibitory effect on skeletal myoblast differentiation under low nutrient conditions, involving AMPK and NAMPT.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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