Thermo Fisher Scientific TCR alpha/beta Monoclonal Antibody (TCR-2), PE

Thermo Fisher Scientific TCR alpha/beta Monoclonal Antibody (TCR-2), PE

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.2 µg/10^6 cells
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• FITC
• Request custom conjugation

Target Information

The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits, all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events.

Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at sites of infection. The subunits of TCR heterodimers are covalently bonded and associate with the CD3 subunits in the endoplasmic reticulum to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease, and mutant self-peptides have been associated with tumors. Engagement of TCRs by bacterial superantigens has been implicated in toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been found in patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), potentially modulating immune responses.

T Cell and TCR Diversity

Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium, showing less TCR diversity and distinct antigen recognition. Subsets of gamma-delta T cells exhibit antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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