Thermo Fisher Scientific IL-16 Monoclonal Antibody (14.1), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

14.1

Immunogen

Recombinant human IL-16

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 4% BSA

Contains

proprietary preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

RRID

AB_223558

Product Specific Information

M161B targets IL-16 in ELISA, and WB applications and shows reactivity with Human samples.

The M161B immunogen is recombinant human IL-16.

M161B detects IL-16 which has a predicted molecular weight of approximately 142 kDa.

The M161B IL16 antibody (biotinylated conjugate of clone 14.1) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M160E (clone 17.1) Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody M161B (clone 14.1) and antibody M160E (clone 17.1) have successfully been used in combination with recombinant IL16 protein SIL16 in ELISA applications.

Target Information

The protein encoded by IL-16 is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of IL-16 undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.