Thermo Fisher Scientific ppNPY Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

5-10 µg/mL

-

Product Specifications

Host/Isotype

Guinea pig / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SDLLMRESTENAPRTR corresponding to residues 76-91 of rat preproneuropeptide Y if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:prepro-Neuropeptide Y,uniProtId:P07808-1,ncbiNodeId:10114,antigenRange:76-91,antigenLength:98,antigenImageFileName:GP14107-150UL_prepro-Neuropeptide_Y_P07808-1_Guinea_pig.svg,antigenImageFileNamePDP:GP14107-150UL_prepro-Neuropeptide_Y_P07808-1_Guinea_pig_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode`s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Note: Sodium azide (NaN3) interferes with peroxidase reactions and should not be used with peroxidase methodologies. If sodium azide is present in any steps of the staining procedure, the tissue should thoroughly be rinsed with sodium azidefree buffer before performing the peroxidase reaction.

Target Information

Neuropeptide Y (NPY) receptors are members of a G protein coupled receptor superfamily and they mediate a variety of physiological responses including feeding and vasoconstriction. The endogenous ligands of NPY receptors are a family of structurally related peptides, including NPY, peptide YY (PYY), and pancreatic polypeptide (PP). Increased activity of NPY and its receptors, Y1R, Y2R, and Y5R has been found in the brain in many forms of experimental obesity. Furthermore, several association studies have supported the association between NPY2R and obesity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.