Thermo Scientific™

Sodium Deoxycholate Detergent

Thermo Scientific Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions.Features of Sodium자세히 알아보기

Thermo Scientific Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions.

Features of Sodium Deoxycholate:

• Popular anionic, bile-acid detergent for many laboratory uses
• Effective in disrupting and dissociating many types of protein interaction
• Can be removed from solution by dialysis
• Useful for elution or regeneration of certain kinds of affinity columns
• High-purity compound with low UV absorptivity

Properties of Sodium Deoxycholate:
• Alternative Names: Sodium deoxycholic acid; deoxycholate, sodium salt
• Chemical Name: 3, 12-α-Dihydroxy-5β-cholan-24-oic acid, monosodium salt
• Molecular Weight: 414.6g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 5 (average)
• Micelle Molecular Weight: 2000g (average)
• Critical Micelle Concentration (CMC): 2 to 6 mM (0.083 to 0.249%, w/v)
• Cloud Point: Unknown
• Dialyzable: Yes

Specifications for Sodium Deoxycholate (Part No. 89904, 89905):
• Formula: C24H39O4Na
• Molecular Weight: 414.6g
• Purity (by HPLC): ≥98%
• Absorbance (1% Detergent Solution): 340nm <0.02; 280nm <0.04; 260nm <0.06
• pH (1% Solution): 5 to 9
• Solubility (in water at 20°C): ≥5%

Sodium deoxycholate (deoxycholic acid) is a water-soluble, bile-acid, ionic detergent commonly used in protein methods. It is most frequently used as a component of cell lysis buffers (e.g., RIPA buffer), but also has been used for liposome preparation, isolation of membrane proteins and lipids, preventing non-specific binding in affinity chromatography and a cell culture media supplement.

The effectiveness of a detergent in any application depend upon the detergent`s concentration. Too much or too little detergent can often have a deleterious effect. It is recommended that you examine a variety of detergent concentrations in your application. At concentrations above 2 mM, cholate will form micelles having MW ∼2000. The small micelle size allows easy removal by dialysis or gel filtration when needed. Note: removal of a detergent from a protein solution may result in protein precipitation and/or aggregation.

Sodium deoxycholate is the detergent recommended for stripping endotoxin (Lipopolysaccharide or LPS) from immobilized Polymyxin B columns. This is the recommended product for use with the Thermo Scientific Detoxi-Gel Endotoxin Removing Gel.

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