Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), PE-Cyanine5.5, eBioscience
인간 CD20을 인식하는 2H7 클론의 마우스 모노클로날 항체로, PE-Cyanine5.5 형광체가 결합되어 있습니다. Flow cytometry용으로 최적화되어 있으며, 5 µL/test 농도로 사용됩니다. B세포 표지 및 활성 연구에 적합하며, 광감도가 있으므로 암실 보관이 필요합니다.
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Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), PE-Cyanine5.5, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.25 µg)/test
- View 12 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine5.5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H7 |
| Conjugate | PE-Cyanine5.5 |
| Excitation / Emission Max | 564 / 692 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_11218092 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV series (395–805)
- Brilliant Violet series (421–711)
- eFluor 450, 506
- FITC
- NovaFluor Blue, Red, Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright series (436–780)
- Request custom conjugation
Product Specific Information
Description:
The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed by developing and mature B cells, but not plasma cells. CD20 may play a role in B-cell activation.
Applications Reported:
Validated for flow cytometric analysis.
Applications Tested:
Pre-titrated and tested on normal human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Light Sensitivity:
Tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples may be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark. Clone-specific performance should be determined empirically.
Excitation/Emission:
488–561 nm / 695 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and malignant B cells, some T lymphocytes, and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 remains on the cell surface after antibody binding, making it an effective target for therapeutic B-cell depletion.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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