
Thermo Fisher Scientific S Tag Polyclonal Antibody
S-Tag 서열(KETAAAKFERQHMDS)에 특이적인 염소 다클론 항체로, WB, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 단백질 발현 검출 및 정제에 활용 가능합니다.
- 카탈로그번호
- A190134A
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific S Tag Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 - 1:30,000 |
| Immunocytochemistry (ICC/IF) | 1:100 - 1:400 |
| ELISA | 1:1,000 - 1:30,000 |
| Immunoprecipitation (IP) | 1 - 4 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | S-Tag (KETAAAKFERQHMDS) conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Goats were immunized with S-Tag (KETAAAKFERQHMDS) conjugated to KLH. The antibody was isolated by affinity chromatography using the peptide immobilized on a solid support.
Target Information
The S-Peptide epitope tag (S-Tag™) is widely used for applications such as localization analysis, expression detection, and purification of polypeptides from mammalian cells.
Its small size (15 amino acids), ability to purify tagged polypeptides without antibodies, and minimal endogenous reactivity in mammalian cells make it an attractive alternative to many existing epitope tags.
Epitope tagging is a versatile strategy for detecting and purifying proteins expressed by cloned genes. Protein expression vectors are engineered with a nucleotide sequence encoding the peptide epitope tag, allowing the protein of interest to be synthesized as a fusion protein. Detection and purification are then mediated by highly specific antibodies to the engineered peptide, eliminating the need for antibodies against each newly cloned protein.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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