Thermo Fisher Scientific IFN gamma Monoclonal Antibody (RMMG-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 39 publications 39 publications

Immunoprecipitation (IP)

Assay-dependent

-

Neutralization (Neu)

Assay-dependent

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Published species

Bacteria, Mouse, Rat

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

RMMG-1

Immunogen

Recombinant mouse IFN-gamma

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-80°C

Shipping conditions

Dry ice

RRID

AB_2536398

Product Specific Information

Binding to mouse IFN-gamma was determined by the method of Scatchard [Scatchard, G. (1949) Ann. N.Y. Acad. Sci. 51, 660-672]. Neutralization was determined by the ability of this antibody to block the activity of mouse IFN-gamma in a cytopathic effect assay using L929 cells challenged with EMCV as described [Rubinstein et al. (1981) J. Vir. 37:755. Staehelin et al. (1981) P.N.A.S. 78(3):1848]

Purity is >95%. Endotoxin level <1 EU/ug.

After receipt, the product may be stored at -20°C for short-term use (less than 6 months). For long-term storage, store the product at -70°C or below for retention of full activity.

Packaged by and distributed for PBL Assay Science.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.