pJTI™ R4 EXP CMV-TO EmGFP pA Vector

The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a자세히 알아보기

The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection, the EmGFP can be inducibly expressed with doxycycline and the successfully retargeted cells will fluoresce green.

Ensure the Success of Your Jump-In™ T-REx™ Retargeting Reactions
Successful retargeting of Jump-In™ T-REx™ parental cell lines like the Jump-In™ T-REx™ HEK293 Kit is dependent on a variety of factors, such as:

• Transfection efficiency
• Cell confluency
• Antibiotic selection conditions
• Quality and concentration of DNA
• Retargeting vector to integrase vector ratio

We strongly recommend including a positive control retargeting reaction using the pJTI™ R4 Exp CMV-TO EmGFP pA vector in your Jump-In™ experiment, along with negative controls (no plasmid DNA, no integrase vector), so you can easily visualize the results and optimize the retargeting conditions.

사양

구성 또는 유도성 시스템Inducible

배달 유형Transfection

유도제Tetracycline

프로모터CMV/TO

제품 유형Mammalian Expression Vector

리포터 유전자GFP (EmGFP)

선택 제제(진핵)Geneticin™ (G-418)

항생제 내성 박테리아Ampicillin (AmpR)

단백질 태그GFP (EmGFP)

수량100 μg

벡터Jump-In Vectors

제품라인Jump-In™

용도(애플리케이션)Stable Cell Line Development, Targeted Integration, Regulated Expression

Unit SizeEach