Thermo Fisher Scientific CD283 (TLR3) Monoclonal Antibody (TLR3.7), Biotin, eBioscience
인간 TLR3(CD283)을 인식하는 Biotin 결합 단클론 항체로, 선천면역 연구에 적합합니다. Flow cytometry 및 세포 내 염색에 검증되었습니다. 0.5 mg/mL 농도의 액상 형태이며, 4°C 암소 보관 권장. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 13-9039-80
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD283 (TLR3) Monoclonal Antibody (TLR3.7), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TLR3.7 |
| Conjugate | Biotin |
| Available Formats | Unconjugated, Functional Grade, PE, Request custom conjugation |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466967 |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The TLR3.7 monoclonal antibody reacts with human Toll-like receptor 3 (TLR3). This receptor family, consisting of at least twelve members, plays a key role in innate immunity by recognizing microbial molecular patterns and inducing antimicrobial responses.
TLR3 recognizes double-stranded RNA (dsRNA), activates NF-kappaB through MyD88-dependent and -independent pathways, and induces type I interferon production.
TLR3.7 has been shown to suppress poly(I):poly(C)-mediated IFN-beta production by human fibroblasts expressing TLR3.
Applications Reported:
TLR3.7 is suitable for flow cytometric analysis. For optimal detection, PE- or Biotin-conjugated TLR3.7 (revealed with SAV PE, Product #12-4312) is recommended.
Applications Tested:
This antibody has been tested by intracellular staining and flow cytometric analysis of A549 and MRC5 cell lines. It can be used at ≤1 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimal titration is recommended for each assay.
Target Information
TLR3 (Toll-like receptor 3, CD283) is a member of the TLR family crucial for pathogen recognition and innate immune activation.
TLRs recognize pathogen-associated molecular patterns (PAMPs) and mediate cytokine production for immune defense.
TLR3 is most abundantly expressed in placenta and pancreas, primarily in dendritic leukocyte subpopulations. It recognizes viral dsRNA, leading to activation of NF-kappaB and type I interferon production.
Unlike other TLRs, TLR3 signals via the TRIF adaptor molecule, activating IRF-3 and NF-kB pathways.
TLR3 is implicated in antiviral defense, recognizing viruses such as Influenza A and West Nile Virus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.