Thermo Fisher Scientific CD1d Monoclonal Antibody (51.1), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 51.1 reacts with human CD1d, a member of the CD1 family similar to non-polymorphic MHC Class I-like molecules. CD1d is a conserved single transmembrane receptor of the Immunoglobulin Superfamily. It can associate with beta-microglobulin or exist independently, suggesting different mechanisms for presenting glycolipid molecules to CD1d-reactive NKT cells.

CD1d expression is found on peripheral B cells, resting monocytes, and cortical thymocytes, with polarized expression on intestinal epithelial cells and low intracellular expression in hepatocytes. In HCV-infected livers, CD1d expression is elevated compared to normal controls.

The 51.1 monoclonal antibody has functional activity, blocking the interaction between CD1d-transfected cells and NKT cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry on normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Light Sensitivity: Protect from light; tandem dye is sensitive to photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be verified empirically.

Excitation: 488–561 nm
Emission: 775 nm
Laser: Blue, Green, or Yellow-Green Laser

Target Information

CD1d is a heavy chain associated with Beta-2 microglobulin on cortical thymocytes. Independent expression has been shown on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of MHC-like glycoproteins. It mediates the presentation of lipid and glycolipid antigens to T cells. The CD1 family genes are clustered on chromosome 1 and differ in localization and ligand specificity. CD1d localizes to late endosomes and lysosomes via a tyrosine-based motif in its cytoplasmic tail. Diseases associated with CD1D include Mycobacterium malmoense infection and autoimmune disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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