Invitrogen™

TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10F` Chemically Competent E. coli

The TA Cloning™ Kit with pCR™2.1 vector provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product자세히 알아보기

The TA Cloning™ Kit with pCR™2.1 vector provides a quick, one-step cloning strategy for directly inserting a _Taq_-amplified PCR product into a plasmid vector. The TA Cloning™ Kit uses the pCR™2.1 cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

Features of the TA Cloning™ Kit with pCR™2.1 vector:
• Fast & convenient—15-minute, room-temperature ligation
• Efficient—blue/white screening and >80% clones with correct insert
• Flexible—choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free—eliminates any enzymatic modifications of the PCR product
• Streamlined—does not require the use of PCR primers that contain restriction sites

The pCR™2.1 vector provides:
• 3`-T overhangs for direct ligation of Taq_-amplified PCR products
• T7 promoter for _in vitro RNA transcription and sequencing
• A versatile polylinker with flanking _Eco_R I sites for easy excision of inserts
• M13 forward and reverse primer sites for sequencing

How TA Cloning™ Works
Taq polymerase has a non-template-dependent activity that adds a single deoxyadenosine (A) to the 3ends of PCR products. The linearized vector supplied in this kit has single 3 deoxythymidine (T) residues. This allows PCR inserts to ligate efficiently with the vector.

Kit Configurations
The TA Cloning™ Kit is offered in a variety of configurations: without competent cells (K2020-20 and K2020-40), with One Shot™ INVFChemically Competent _E. coli_ (K2000-01 and K2000-40), with One Shot™ TOP10F Chemically Competent E. coli (K2030-01 and K2030-40), and with One Shot™ TOP10 Chemically Competent E. coli (K2040-01 and K2040-40) in 20- and 40-reaction kit sizes.

사양

박테리아 또는 효모 균주TOP10F ́

세포 유형Chemically Competent E. coli

클로닝 방법TA Cloning

형식Kit

용도(애플리케이션)PCR Cloning

반응 수20 Reactions

제품라인One Shot™

제품 유형Cloning Kit

프로모터T7

수량20 reactions

벡터pCR2.1

Unit SizeEach