Thermo Fisher Scientific KAP-1 Polyclonal Antibody
KAP-1 단백질을 인식하는 염소 폴리클로날 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. 인간 KRAB-Associated Protein 1의 N-말단(1–50 aa)을 면역원으로 제작되었으며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of human KRAB-Associated Protein 1 (TRIM28, UniProt ID: Q13263-1, antigen length: 835 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The KRAB (Kruppel-associated box) domain is approximately 45 amino acids long and functions as a transcriptional repression domain found in many transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription via interactions with KAP-1 (KRAB-associated protein 1).
KAP-1 is an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger. It forms complexes with KRAB-domain transcription factors to enhance transcriptional repression and interacts with HP1 (heterochromatin protein 1) and KRAZ1. KAP-1 targets KRAZ1 to centromeric heterochromatin containing HP1α, thereby regulating transcriptional repression. Mutant KAP-1 proteins that bind KRAB but not HP1 lead to random KRAZ1 distribution and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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