
Thermo Fisher Scientific S-peptide Epitope Tag Polyclonal Antibody
S-peptide 태그 인식 토끼 폴리클로날 항체로, Western blot, ICC/IF, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 고농도(1.34 mg/mL) 액상 형태. 단백질 발현 검출 및 정제 연구용으로 최적화.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Immunoprecipitation (IP) | 2.5 µg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | The immunogen used to generate this antibody corresponds to S Tag (KETAAAKFERQHMDS). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.34 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913168 |
Product Specific Information
Store as concentrated solution. Centrifuge briefly prior to opening vial.
For short-term storage (1–2 weeks), store at 4°C.
For long-term storage, aliquot and store at -20°C or below.
Avoid multiple freeze-thaw cycles.
Target Information
The S-Peptide epitope tag (S-Tag™) is widely used for localization analysis, expression detection, and purification of polypeptides from mammalian cells.
Its small size (15 amino acids), ability to purify tagged proteins without antibodies, and minimal cross-reactivity with endogenous mammalian proteins make it an excellent choice among epitope tags.
Epitope tagging enables detection and purification of proteins expressed by cloned genes.
Protein expression vectors are engineered to include a nucleotide sequence encoding the peptide tag, producing a fusion protein upon expression.
Detection and purification are then mediated by specific antibodies to the tag, eliminating the need for target-specific antibodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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