Thermo Fisher Scientific CD80 Monoclonal Antibody (OTI1E9), TrueMAB
CD80 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, ICC/IF, Flow Cytometry에 사용 가능. Human, Dog, Rat 반응성. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로 T 세포 활성화 관련 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E9 |
| Immunogen | Full length human recombinant protein of human CD80 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152).
Both are expressed on professional antigen-presenting cells and play essential roles in T cell activation.
CD80 is rapidly induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, follicular B lymphoma cells, T cells, and monocytes.
CD80 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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