Invitrogen™

Fast SYBR™ Green Cells-to-CT™ Kit

Ambion™ Fast SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to자세히 알아보기

Ambion™ Fast SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results. A breakthrough cell lysis and RNA stabilization technology eliminates the laborious and time-consuming RNA purification process completely. The kit includes sufficient reagents for 400 reactions. The Fast SYBR™ Green Cells-to-CT™ Kit contains AmpliTaq™ Fast DNA Polymerase, UP, which allows instant hot start, thus minimizing non-specific product formation with fast real-time PCR.

• Includes optimized reagents to work efficiently, right out of the box
• Simple, effective Cells-to-CT™ technology enables sample preparation at room temperature in only 7 minutes, including DNase treatment
• Validated accuracy, exceptional reproducibility, and maximal sensitivity from 10 to 100,000 cells per sample; results equivalent to those from purified RNA

Extraordinary Value
The kit includes reagents for sample preparation integrated into a complete, optimized gene expression workflow including reverse transcription reagents and a high-performance PCR master mix with SYBR™ Green.

Extraordinary Ease
The protocol begins with a simple 7-minute sample preparation procedure. First, cells are washed in PBS, and then lysed for 5 minutes at room temperature; DNase treatment can be performed concurrently. The lysis reaction is stopped, and the sample is then ready for reverse transcription or storage at –20°C for up to 5 months. Because samples are processed directly in culture plates (96- or 384-well), sample handling and the potential for sample loss or transfer error are minimized, resulting in higher reproducibility. Following sample preparation, a portion of the cell lysate is added to an RT reaction followed by real-time PCR using the included master mix.

Extraordinary Performance
To demonstrate the performance of the kit, replicate experiments were run using either traditional RNA purification and real-time PCR analysis, or the FastSYBR™ Green Cells-to-CT™ Kit. The kit shows equivalent performance to results obtained using purified RNA for analysis of 10 to 100,000 cells. In addition, the sensitivity, efficiency, and dynamic range were shown to be superior to competitor lysate kits. Samples prepared with the kit have been tested for compatibility on various real-time PCR platforms, including those from Applied Biosystems™, Roche, and Bio-Rad.

Accessory Product:
The SYBR™ Green Cells-to-CT™ Control Kit (Cat. No. 4402959) contains a Xeno™ RNA control and primer set and is recommended to monitor the efficiency of cell lysis and amplification inhibition in Cells-to-CT™ reactions.

사양

검출 방법SYBR

중합효소AmpliTaq Fast DNA Polymerase

반응 속도Fast

용도(장비)7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode

그린 기능Fewer resources used and less waste, Less hazardous

라벨 또는 염료SYBR Green

패시브 참조 염료ROX (Pre-mixed)

제품라인Cells-to-CT™, SYBR™

정제 시간5 min.

순도 또는 품질 등급UP (Ultra Pure)

수량400 reactions

역전사 효소M-MLV

배송 조건Dry Ice

용도(애플리케이션)RT-PCR

농도20X

핫 스타트Built-In Hot Start

반응 수400 reactions

샘플 종류Cells

Unit SizeEach