Thermo Fisher Scientific Phosphotyrosine (P-Tyr) Monoclonal Antibody (PY793)
Phosphotyrosine (P-Tyr) 단백질 검출용 마우스 단클론 항체. IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. 높은 특이성과 재현성을 제공하며, 연구용으로 적합. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PY793 |
| Immunogen | Phosphotyrosine conjugated to BSA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Tyrosine phosphorylation plays a key role in mitogenic signal transduction from transmembrane receptors and in oncogene tyrosine kinase-mediated transformation. Phosphorylation of specific tyrosine residues is a primary mechanism in normal mitogenesis, cell cycle progression, and oncogenic transformation. Its involvement in differentiation and gap junction communication remains an active research area.
Antibodies that specifically recognize phosphorylated tyrosine residues are invaluable tools for studying tyrosine-phosphorylated proteins and their biochemical pathways. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is particularly useful for detecting P-Tyr proteins in immunohistochemical and immunocytochemical assays, where the use of a secondary antibody may complicate detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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