Thermo Fisher Scientific Nitrotyrosine Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:2,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

1:200-1:2,000

View 27 publications 27 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 10 publications 10 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 5 publications 5 publications

ELISA (ELISA)

-

View 3 publications 3 publications

Radioimmune Assays (RIA)

-

View 1 publication 1 publication

Competition Assay (CA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Keyhole limpet hemocyanin (KLH) containing nitrated tyrosines

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_221457

Target Information

Nitrotyrosine is a relatively stable product formed from various reaction pathways. Perhaps most notable is the reaction of peroxynitrite (formed from superoxide and nitric oxide (NO) radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration reactions on proteins resulting in inactivation of certain housekeeping enzymes (e. g., alpha1-antiproteinase) as well as endogenous antioxidant enzymes such as catalase and superoxide dismutase (SOD). Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Nitrotyrosine has been implicated in the pathogenesis of several inflammatory, infectious, and degenerative human diseases, such as Alzheimers and Parkinsons disease, multiple sclerosis, amyotrophic lateral sclerosis (ALS), asthma, atherosclerosis, and a variety of conditions precipitated by endothelial injury. At the protein level, tyrosine nitration can lead to loss or alteration of protein function, as demonstrated for the oncogenic protein p53 and the mitochondrial protein, manganese-dependent superoxide dismutase (MnSOD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.