Thermo Fisher Scientific Nitrotyrosine Polyclonal Antibody
Nitrotyrosine 검출용 Rabbit Polyclonal Antibody로 다양한 종에서 반응. Western blot, IHC 등 여러 응용 가능. KLH 기반 nitrated tyrosine을 면역원으로 사용. 순도 높은 액상 형태, 4°C 보관. 세포 손상 및 염증 연구용 마커로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:2,000 | 17 |
| Immunohistochemistry (IHC) | 1:200–1:2,000 | 27 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 10 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 2 |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 5 |
| ELISA | - | 3 |
| Radioimmune Assay (RIA) | - | 1 |
| Competition Assay (CA) | - | 1 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Keyhole limpet hemocyanin (KLH) containing nitrated tyrosines |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient |
| RRID | AB_221457 |
Target Information
Nitrotyrosine is a relatively stable product formed from various reaction pathways, notably the reaction of peroxynitrite (formed from superoxide and nitric oxide radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration on proteins, leading to inactivation of certain enzymes such as alpha1-antiproteinase, catalase, and superoxide dismutase (SOD).
Nitrotyrosine serves as an indicator of cell damage, inflammation, and nitric oxide production. It is implicated in the pathogenesis of several inflammatory, infectious, and degenerative diseases, including Alzheimer’s, Parkinson’s, multiple sclerosis, amyotrophic lateral sclerosis (ALS), asthma, and atherosclerosis. Tyrosine nitration may alter protein function, as observed in proteins such as p53 and MnSOD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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