Invitrogen™

Ambion™ RNase I, cloned, 100 U/μL

Ambion™ RNase I efficiently cleaves after all four bases of single-stranded RNA, in contrast to RNase A, which only cleaves자세히 알아보기

Ambion™ RNase I efficiently cleaves after all four bases of single-stranded RNA, in contrast to RNase A, which only cleaves after C and U residues. RNase I degrades all RNA dinucleotide bonds leaving a 5hydroxyl and 2, 3` cyclic monophosphate. Supplied in one tube of 25,000 U (100 U/ μL). RNase I will degrade any RNA to a mixture of mononucleotides, dinucleotides, and trinucleotides and does not degrade DNA, although it will bind to DNA. It has a marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in ribonuclease protection assays. This RNase I is rigorously tested for nonspecific contaminating endonuclease, exonuclease, and protease activity.

Unit Definition:
One unit is the amount of enzyme required to produce 1 μg of acid-soluble material from mouse liver RNA in 30 min at 37°C.

사양

농도100 U/μL

배송 조건Dry Ice

효소RNase

수량10,000 U

제품 유형RNase I

제품라인Ambion™

Unit SizeEach