Thermo Fisher Scientific MAPK14 Monoclonal Antibody (3D5)
MAPK14(p38α) 단백질을 표적하는 Mouse monoclonal antibody(3D5). WB, IHC, ELISA, IP, PLA 등 다양한 응용에 적합. Human 반응성, Affinity chromatography로 정제된 액상형 항체. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| ELISA | 10 µg/mL |
| Immunoprecipitation (IP) | 1–5 µg/mL |
| in situ PLA (PLA) | 1:1200 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D5 |
| Immunogen | MAPK14 (AAH31574, 260 a.a. approximately 360 a.a) partial recombinant protein with GST tag (MW of GST tag alone: 26 KDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
QSLTQMPKMN FANVFIGANP LAVDLLEKML VLDSDKRITA AQALAHAYFA QYHDPDDEPV ADPYDQSFES RDLLIDEWKS LTYDEVISFV PPPLDQEEME S
Target Information
MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates various cellular responses to stress, inflammation, and other signals.
p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motif.
There are four isoforms of p38 MAPK, differing in tissue expression and affinity for upstream activators and downstream effectors.
When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation.
Activated MAPK kinase-3/6 phosphorylate Thr180 and Tyr182 residues in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2, and other transcription factors through phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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