Thermo Fisher Scientific Human IL-9, Animal-Free Recombinant Protein, PeproTech
Human IL-9 recombinant protein, animal-free, expressed in E. coli. High purity (≥98%) and low endotoxin (<0.1 EU/µg). Functional activity confirmed via MO7e cell proliferation assay. Suitable for research use in cytokine signaling and Th2/Th9 cell stud...
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Applications
- Functional Assay (Functional): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | MQGCPTLAGI LDINFLINKM QEDPASKCHC SANVTSCLCL GIPSDNCTRP CFSERLSQMT NTTMQTRYPL IFSRVKKSVE VLKNNKCPYF SCEQPCNQTT AGNALTFLKS LLEIFQKEKM RGMRGKI |
| Molecular Weight | 14 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥ 98% by SDS-PAGE gel and HPLC analyses |
| Endotoxin Concentration | < 0.1 EU/µg |
| Activity | Stimulates proliferation of human MO7e cells; expected ED50 ≤ 0.2 ng/ml (specific activity ≥ 5 × 10⁶ units/mg) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
Recombinant Human IL-9 is a 14.0 kDa protein consisting of 127 amino acid residues, including 10 cysteine residues that are fully conserved between human and murine proteins.
This product is shipped at ambient temperature. For storage, handling, and reconstitution information, refer to the lot-specific Certificate of Analysis.
Target Information
Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. It regulates various cell types involved in Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells.
Mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein (14 kDa). Native mouse IL-9 is heavily glycosylated.
TGF-β plays a key role in differentiating IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby promoting Th9 cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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