Thermo Fisher Scientific GFP Polyclonal Antibody
GFP 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC, ICC 등 다양한 응용에 사용 가능. 2 mg/mL 농도의 액상 항체로, PBS(pH 7.2)에 5mM sodium azide 포함. 4°C 보관, 형광 단백질 연구용에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 | 375 |
| Immunohistochemistry (IHC) | 1:200–1:2,000 | 942 |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | 33 |
| Immunohistochemistry (Frozen) (IHC (F)) | – | 50 |
| Immunohistochemistry - Free Floating (IHC (Free)) | – | 12 |
| Immunocytochemistry (ICC/IF) | 1:200–1:2,000 | 365 |
| Flow Cytometry (Flow) | – | 14 |
| ELISA | – | 1 |
| Immunoprecipitation (IP) | – | 82 |
| ChIP assay (ChIP) | – | 57 |
| Neutralization (Neu) | – | 1 |
| Functional Assay | – | 1 |
| in situ PLA (PLA) | – | 5 |
| Immunomicroscopy (IM) | – | 7 |
| Inhibition Assays (IA) | – | 1 |
| Gel Shift (GS) | – | 1 |
| RNA Immunoprecipitation (RIP) | – | 3 |
| Affinity Purification (AP) | – | 5 |
| In vitro Assay (IV) | – | 2 |
| GST Pull Down (GPD) | – | 2 |
| Miscellaneous PubMed (Misc) | – | 93 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GFP isolated from Aequorea victoria (jellyfish) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_221569 |
Product Specific Information
- Protect from light.
- When stored undiluted at 2–8°C, product is stable for at least 3 months.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light during bioluminescence. GFP is widely used as a reporter protein for gene expression and as a protein tag in both eukaryotic and prokaryotic organisms.
As a fusion tag, GFP enables localization studies, tracking of protein movement, and analysis of subcellular compartment dynamics. GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically. It absorbs blue light (395 nm) and emits green light (508 nm) without the need for additional cofactors.
GFP fluorescence is stable, species-independent, and suitable for diverse applications such as gene expression monitoring, protein localization, protein-protein interaction studies (e.g., yeast two-hybrid), and FRET-based distance measurements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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