Thermo Fisher Scientific NLRP3 Monoclonal Antibody (25N10E9)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-5 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

25N10E9

Immunogen

Recombinant mNLRP3 NOD domain protein (AA 216-385). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NLRP3,uniProtId:Q8R4B8-1,ncbiNodeId:10090,antigenRange:216-385,antigenLength:1033,antigenImageFileName:MA5-16274_NLRP3_Q8R4B8-1_House_mouse.svg,antigenImageFileNamePDP:MA5-16274_NLRP3_Q8R4B8-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537791

Target Information

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome, and neonatal-onset multisystem inflammatory disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5 UTR splice patterns are biologically valid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.