Invitrogen™

PureLink™ Microbiome DNA Purification Kit

The PureLink™ Microbiome DNA Purification Kit enables fast purification of high-quality microbial and host DNA from a wide variety of자세히 알아보기

The PureLink™ Microbiome DNA Purification Kit enables fast purification of high-quality microbial and host DNA from a wide variety of sample types, including challenging samples such as stool and soil. The kit uses proven PureLink spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing, or other applications. The highly efficient triple lysis approach, fast removal of inhibitors, and versatility make this the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.

Features of the PureLink Microbiome DNA Purification Kit include:

• Efficient lysis of all microorganisms (including durable species with thicker and more complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
• Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
• Streamlined protocols for numerous biological samples
• Recovery of high-purity DNA compatible with common downstream applications such as qPCR and next-generation sequencing

One kit to isolate microbial and host DNA from a diverse set of sample types
The PureLink Microbiome DNA Purification Kit minimizes the need to order individual kits for specific sample types because it has been optimized for use with a wide range of biological samples.

The kit enables microbial—and, where applicable, host—DNA purification from the following samples:

• Stool
• Urine
• Saliva
• Swabs (vaginal, buccal, skin, rectal, environmental)
• Transport media
• Growth media
• Soil

Analyzing the human microbiome
The human body is populated by 100 trillion bacteria, archaea, fungi, protists, and viruses, all of which play a fundamental role in our well-being. The term microbiome refers to the various different microbes in those communities. Presently, their genes are the most straightforward method of identifying these organisms. Deviations from healthy microbial compositions have been linked to many human diseases, including inflammatory bowel disease, obesity, cancer, asthma, diabetes, and allergies.

Purification of the aggregate microbial DNA in a biological sample is a key step in understanding how the microbiome influences human health. It is of utmost importance that the DNA recovered accurately reflects the representation of the diverse microbes in the sample community. The mechanical disruption and triple lysis approach used in the PureLink Microbiome DNA Kit help ensure that microbial DNA from even the toughest samples, such as gram-positive bacteria found in stool, can be recovered—allowing you to identify the full spectrum of microbes present.

사양

컬럼 유형Spin Column

Isolation TechnologySilica Spin Column

스케일Mini

샘플 종류Microbial Culture, Saliva, Soil, Stool, Host DNA from Stool, Swabs (Buccal, Vaginal, Skin, Rectal, or Environmental), Cells, Food and Environmental

용리량50 to 200 μL

최종 제품 유형Microbial and host DNA

용도(애플리케이션)PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization

고처리량 호환성Not High-throughput Compatible (Manual)

수량50 Preps kit

배송 조건Room Temperature

테스트 시간Up to 55 min. (Including sample homogenization)

수율≤25 μg

시작 물질 양Buccal/Vaginal/Skin/Rectal/Environmental Swab: 1 swab
Microbial Culture: ≤5 mL
Saliva: ≤2 mL
Soil: ≤2 g
Stool: ≤2 g
Urine: ≤10 mL

Unit SizeEach