Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP
HRP 결합 Donkey anti-Mouse IgG (H+L) 고도로 교차흡착된 2차 항체로 Western blot, IHC, ELISA에 적합. 다양한 포맷 제공으로 높은 특이성과 낮은 교차반응 보장. 연구용으로 높은 감도와 신뢰성 제공.
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- A16017
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Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2534691 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Rehydrate with 1.1 mL of deionized water and let stand for 30 minutes at room temperature to dissolve. Product is overfilled to ensure complete recovery. Centrifuge to remove particulates. Prepare fresh working dilution daily. Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin mouse serum immunoglobulins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, or sheep.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. Useful for detection, sorting, or purification of target proteins.
Secondary antibodies provide versatility for multiple detection systems (HRP, AP, fluorescence) and enhance sensitivity through signal amplification.
Typically generated by immunizing host animals with pooled immunoglobulins from the target species and purified via immunoaffinity chromatography or other modifications for high specificity.
Available Formats
- Alexa Fluor 350
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Alexa Fluor Plus 680
- Alexa Fluor Plus 800
- AP
- Biotin
- FITC
- TRITC
- Request custom conjugation available
Usage Note
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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