Thermo Scientific™

Z`-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is자세히 알아보기

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7 and a consensus seq Hyd-X-R-X-X-S/T-X-X, where Hyd is bulky hydrophobic, F > L > V >> A, and X is any residue.

사양

제품라인Z ́-LYTE™

어세이Serine/Threonine Kinase Assay

어세이 입력Biochemical phospho-specific activity (no antibody)

여기/방출405/465, 535 nm

형식384-well Plate

용도(애플리케이션)FRET

라벨 또는 염료Methylcoumarin

반응 수800 Reactions

수량800 x 20 μL Assays

판독End Point

참조Biochem J. vol 296, pp. 843-9 (1993), J. Biol Chem, vol. 260, pp 14471-6 (1985)

배송 조건Dry Ice

소스Ser 7 Glycogen Synthase (KKLNRTLSVA) Based

기질 특성Peptide-Based Substrate

타겟 입력MAPK8, MAPKAPK2, MAPKAPK5, CAMK2D, MAPK9, STK22B, STK22D, CAMK2A, MAPK14, MAPK10, MAPKAPK3

타겟 효소Serine/Threonine Kinases

검증된 키나아제MAPKAPK2, MAPKAPK3, MAPKAPK5, STK22B, STK22D, CAMK2A, CAMK2D, MAPK8, MAPK9, MAPK10, MAPK14

Unit SizeEach