Gibco™

DMEM/F-12, GlutaMAX™ supplement

DMEM/F-12 (Dulbecco`s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different자세히 알아보기

DMEM/F-12 (Dulbecco`s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM / F-12 is modified as follows:

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Hams F-12. This formulation combines DMEMs high concentrations of glucose, amino acids, and vitamins with F-12`s wide variety of components. DMEM/F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM/F-12 product made in our Scotland facility (31331-028). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.

This product is not used for in vitro diagnostic purpose in some countries.

사양

분류Animal Origin-free

농도1 X

Culture TypeMammalian Cell Culture

형태Liquid

제품 유형DMEM (Dulbecco`s Modified Eagle Medium)/F-12

유통 기한12 Months

멸균Sterile-filtered

Sterilization MethodSterile-filtered

첨가제 포함GlutaMAX, High Glucose, Phenol Red, Sodium Pyruvate

첨가제 없음No HEPES

제조 품질cGMP-compliant under the ISO 13485 standard

수량500 mL

Unit SizeEach