Thermo Fisher Scientific Nanog Polyclonal Antibody
Nanog 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 폴리클로날 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합합니다. 인간 및 돼지 반응성이 확인되었으며, 줄기세포 연구 및 분화 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.03–0.1 µg/mL | - |
| Immunohistochemistry (IHC) | Assay-dependent | 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Pig |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (QNQRMKSKRWQKNN) corresponding to the internal amino acids of NANOG (aa 144–158) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10985008 |
Product Specific Information
- Predicted to react with canine based on sequence homology.
- Successfully tested in a sandwich ELISA as both a capture and detection antibody in combination with PA5-18678.
Target Information
NANOG (Nanog homeobox) is a divergent homeodomain protein that regulates pluripotency and differentiation in undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines but absent in differentiated cells. Human NANOG protein shares 52% amino acid identity with the mouse protein and 85% identity in the homeodomain.
Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to 6F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Studies show high levels of NANOG expression in undifferentiated N-Tera embryonal carcinoma cell lines.
NANOG functions as a transcription regulator involved in embryonic stem (ES) cell proliferation and self-renewal. Its role in embryonic development suggests potential applications in generating stem cells for regenerative medicine and degenerative disease treatment.
Induced pluripotent stem (iPS) cells can be generated by expressing transcription factors such as NANOG, Sox2, Klf4, Lin28, and c-Myc. Overexpression of NANOG promotes S-phase entry and proliferation. Dysregulation of NANOG is associated with teratocarcinoma and germ cell/embryonal cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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