Thermo Fisher Scientific SELK Polyclonal Antibody
SELK 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 인간 시료 반응성, 항원 친화 크로마토그래피로 정제. 액상 형태로 4°C 또는 -20°C 보관 가능.
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Thermo Fisher Scientific SELK Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human SELK. Recombinant protein control fragment (Product #RP-88720). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2647098 |
Product Specific Information
Immunogen sequence:
KRRSYGNSSD SRYDDGRGPP GNPPRRMGRI NHLRGPS
Highest antigen sequence identity to the following orthologs:
- Mouse: 92%
- Rat: 92%
Target Information
SELK is a selenoprotein containing a selenocysteine residue at its active site. It is localized to the endoplasmic reticulum and highly expressed in the heart, where it may function as an antioxidant. SELK interacts with ER-associated degradation (ERAD) components such as p97 ATPase, Derlin-1 and -2, and SelS, as well as proteins in the oligosaccharyltransferase complex, suggesting a role in Derlin-dependent ERAD of glycosylated misfolded proteins. SELK also contributes to Ca²⁺ flux in immune cells and is cleaved by m-calpain in resting macrophages; this cleavage is inhibited upon macrophage activation through Toll-like receptors.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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