Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Violet 786, eBioscience
인간 CCR7(CD197)을 인식하는 Rat IgG2a 단클론 항체로, Brilliant Violet 786 형광체가 결합된 제품입니다. Flow Cytometry용으로 최적화되어 있으며, 높은 감도와 안정성을 제공합니다. 4°C 암조건 보관, 비동결 권장.
- 카탈로그번호
- 417-1979-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Violet 786, eBioscience
Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Violet 786, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (1.0 µg)/test
Publications: -
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Violet™ 786, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D12 |
| Conjugate | Brilliant Violet™ 786 |
| Excitation / Emission Max | 406 / 788 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2937223 |
Additional Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 661 / 737
- Brilliant Violet 421 / 480 / 711
- FITC
- NovaFluor Red 685 / 700 / 755
- NovaFluor Yellow 700
- PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600 / 645 / 780
- Custom conjugation available
Product Specific Information
The 3D12 monoclonal antibody reacts with human CCR7 (CD197), a G-protein-coupled chemokine receptor for CCL19 and CCL21. CCR7 is expressed on T cells and dendritic cells (DCs), distinguishing naive and memory T cell subsets and mediating migration to lymphoid tissues.
- Optimal Staining: Incubate ≥45 minutes at 2–8°C.
- Applications Reported: Flow cytometric analysis.
- Applications Tested: Pre-diluted and validated on normal human peripheral blood cells.
- Recommended Use: 5 µL (1.0 µg) per test (100 µL final volume).
- Cell Count Range: 10⁵–10⁸ cells/test.
Brilliant Violet™ 786 (BV786) emits at 786 nm and is intended for use with violet (405 nm) laser-equipped cytometers.
Use Super Bright Complete Staining Buffer or Brilliant Stain Buffer™ to minimize non-specific polymer interactions.
Light-sensitive; protect from light.
Fixation Compatibility:
- Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution (up to 3 days at 4°C, dark).
- Not compatible with methanol-based fixation.
Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
CCR7 is a G-protein-coupled receptor induced by Epstein Barr virus (EBV). It mediates B lymphocyte activation and is expressed in blood, bone marrow, lymph node, and intestine. CCR7 regulates T cell migration to inflamed tissues and dendritic cell maturation. Ligands include CCL19 (ECL) and CCL21.
CCR family members (CCR1–CCR5, Duffy antigen) are key in chemotaxis and immune cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.