Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Violet 786, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (1.0 µg)/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 661 / 737
• Brilliant Violet 421 / 480 / 711
• FITC
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 700
• PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600 / 645 / 780
• Custom conjugation available

Product Specific Information

The 3D12 monoclonal antibody reacts with human CCR7 (CD197), a G-protein-coupled chemokine receptor for CCL19 and CCL21. CCR7 is expressed on T cells and dendritic cells (DCs), distinguishing naive and memory T cell subsets and mediating migration to lymphoid tissues.

• Optimal Staining: Incubate ≥45 minutes at 2–8°C.
• Applications Reported: Flow cytometric analysis.
• Applications Tested: Pre-diluted and validated on normal human peripheral blood cells.
• Recommended Use: 5 µL (1.0 µg) per test (100 µL final volume).
• Cell Count Range: 10⁵–10⁸ cells/test.

Brilliant Violet™ 786 (BV786) emits at 786 nm and is intended for use with violet (405 nm) laser-equipped cytometers.
Use Super Bright Complete Staining Buffer or Brilliant Stain Buffer™ to minimize non-specific polymer interactions.
Light-sensitive; protect from light.

Fixation Compatibility:

• Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution (up to 3 days at 4°C, dark).
• Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CCR7 is a G-protein-coupled receptor induced by Epstein Barr virus (EBV). It mediates B lymphocyte activation and is expressed in blood, bone marrow, lymph node, and intestine. CCR7 regulates T cell migration to inflamed tissues and dendritic cell maturation. Ligands include CCL19 (ECL) and CCL21.
CCR family members (CCR1–CCR5, Duffy antigen) are key in chemotaxis and immune cell migration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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