
Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, PE
PE 형광 표지된 Goat anti-Rat IgG (H+L) F(ab')2 2차 항체로, Flow Cytometry에 최적화되어 있습니다. Rat IgG에 특이적이며, Mouse IgG와의 교차 반응이 최소화되었습니다. 동결건조 형태로 제공되며, 재구성 후 4°C 암소 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 1:5–1:10 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Whole rat IgG |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Purified |
| Storage Buffer | PBS with 1% BSA, 5% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550667 |
| Target | IgG |
| Cross Adsorption | Against mouse IgG |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
F(ab`)2 fragments were prepared by pepsin digestion. Cross-reactivity with mouse IgG has been reduced by adsorption.
For FACS analysis, use 50 µL of the suggested working dilution to label 1×10^6 cells in 100 µL.
Reconstitute with 0.5 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility, enabling users to use many detection systems (e.g., HRP, AP, fluorescence). They also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Conjugate Formats
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- AP, APC, Biotin, Cyanine3, Cyanine5, DSB-X Biotin
- DyLight 488, 550, 594, 650, 680, 755, 800
- FITC, HRP, Texas Red-X, TRITC
- Custom conjugation available upon request
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