Thermo Fisher Scientific CDKN2A Monoclonal Antibody (OTI5H8), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, it is recommended to add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The CDKN2A gene produces several transcript variants differing in their first exons. At least three alternatively spliced variants encode distinct proteins, two of which act as inhibitors of CDK4 kinase. Another transcript variant encodes an alternate open reading frame (ARF) protein that stabilizes the tumor suppressor p53 by interacting with and sequestering MDM2, an E3 ubiquitin-protein ligase responsible for p53 degradation.
Despite structural and functional differences, all isoforms contribute to G1 cell cycle regulation through CDK4 and p53 pathways. Mutations or deletions in this gene are frequently observed in various tumors, confirming its role as a key tumor suppressor gene.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.