Thermo Fisher Scientific SIRT1 Polyclonal Antibody
Thermo Fisher Scientific의 SIRT1 Polyclonal Antibody는 인간 SIRT1 단백질을 인식하는 고품질 토끼 IgG 항체로, Western blot, ELISA, IP에 적합합니다. Affinity chromatography로 정제되었으며, 안정적인 액상 형태로 연구용으로 제공됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1.0 µg/mL |
| ELISA | 5.0 µg/mL |
| Immunoprecipitation (IP) | 1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified Anti-SIRT1 antibody prepared from whole rabbit serum immunized with a synthetic peptide near the C-terminal of human SIRT1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.07 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-SIRT1 is directed against human SIRT1. BLAST analysis suggests reactivity with SIRT1 from human, mouse, and rat based on 100% homology for the immunizing sequence.
Target Information
NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and coordinates several cellular functions such as cell cycle, DNA damage response, metabolism, apoptosis, and autophagy.
SIRT1 modulates chromatin function through histone deacetylation and promotes alterations in histone and DNA methylation leading to transcriptional repression.
It plays an essential role in skeletal muscle differentiation and mediates the inhibitory effect on skeletal myoblast differentiation under low nutrient conditions involving AMPK and NAMPT.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송 정보
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