Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Brilliant Ultra Violet 395, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Recommended Dilution

• 1.0 µg/test

Product Specifications

Additional Formats

• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 647
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 563
• Brilliant UV 615
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 711
• eFluor 450
• eFluor 506
• FITC
• Functional Grade
• Pacific Orange
• PE
• PE-Cyanine5
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780

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Product Specific Information

This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA).
KLRG1 is a homodimer of glycosylated 30–38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM).
This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes (≤1.0 µg/test)
• Recommended Volume: 100 µL per test (10⁵–10⁸ cells/test)

Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is intended for use on cytometers equipped with a 355 nm ultraviolet laser.
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family.
It contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic domains, which interact with SH2 domains of phosphatases such as SHP-1.
KLRG1 is an inhibitory receptor expressed on NK cells and certain T cells, involved in lysis of tumor and virus-infected cells, and regulation of immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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