Gibco™

DMEM, low glucose, pyruvate, HEPES

Dulbecco`s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.자세히 알아보기

Dulbecco`s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco™ DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle`s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco™ DMEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers a comparable Gibco™ DMEM product made in our Grand Island facility (12320-032). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

사양

배양 환경CO2

분류Animal Origin-free

농도1 X

Culture TypeMammalian Cell Culture

형태Liquid

제품 유형Dulbecco`s Modified Eagle Medium (DMEM)

Serum LevelStandard Serum Supplementation

유통 기한12 Months

멸균Sterile-filtered

첨가제 포함Low Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate

제조 품질cGMP-compliant under the ISO 13485 standard

삼투압250 - 290 mOsm/kg

pH7 to 7.4

제품라인Gibco™

수량500 mL

배송 조건Room Temperature

Unit SizeEach