Thermo Fisher Scientific Human PRKCI (PKC iota), His Tag Recombinant Protein

Thermo Fisher Scientific Human PRKCI (PKC iota), His Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution

• Assay-dependent

Product Specifications

Product Specific Information

• Spin briefly before use for maximum recovery.
• Aliquoting of 5 µg, 10 µg, and 20 µg sizes is not recommended; use directly from original packaging.
• For larger sizes, aliquoting may reduce freeze/thaw cycles; avoid aliquots below 20 µL.
• Do not store diluted kinase.
• When stored at -80°C, product is guaranteed for 6 months from date of purchase.

Protein Form: Recombinant, Full Length
Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical binding/kinetic
System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Z'-LYTE™, Omnia™ Bulk Substrate

Target Information

The PKC family of serine/threonine kinases, including PRKCI (PKC iota), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified, differing in structure, tissue distribution, localization, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family, which is Ca²⁺- and phospholipid-dependent.
PRKCI is calcium-independent and phospholipid-dependent, not activated by phorbol esters or diacylglycerol.
It interacts with GTPase RAB2, phosphorylates GAPDH, and is involved in microtubule dynamics in the early secretory pathway.
PRKCI contributes to BCL-ABL-mediated resistance to apoptosis, protecting leukemia cells against drug-induced cell death.
A single exon pseudogene is mapped on chromosome X.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.