Thermo Fisher Scientific Human KIT, His Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution: Assay-dependent
Publications: Refer to Thermo Fisher Scientific documentation.

Product Specifications

Product Specific Information

For maximum recovery, spin prior to use.
Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use materials in original packaging until exhausted.
For larger sizes, aliquoting can reduce freeze/thaw cycles — do not prepare aliquots below 20 µL.
Never store kinase diluted.
Properly stored at -80°C, product is guaranteed for 6 months from date of purchase.

Protein Form: Cytoplasmic

Target Information

KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for mast cell growth factor (MGF or stem cell factor, SCF). It regulates growth and activation of various hematopoietic and non-hematopoietic cells.
Mutations in KIT are linked to gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
The activation loop mutation D816V is a key therapeutic target for neoplastic mast cell growth.
c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract Cajal cells.
Upon SCF binding, c-kit dimerizes, leading to receptor activation and autophosphorylation of tyrosine residues (e.g., Tyr703), enabling Grb2 docking and Ras/ERK pathway activation.
SCF/c-kit signaling also activates PI3K, PLC-γ, and JAK/STAT pathways, essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.