Thermo Fisher Scientific GCAT Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 170-419 of human GCAT (NP_0551061) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GCAT,uniProtId:O75600-1,ncbiNodeId:9606,antigenRange:170-419,antigenLength:419,antigenImageFileName:PA5-90717_GCAT_O75600-1_Rabbit.svg,antigenImageFileNamePDP:PA5-90717_GCAT_O75600-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.67 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806199

Product Specific Information

Immunogen sequence: KAHKYRYRHL DMADLEAKLQ EAQKHRLRLV ATDGAFSMDG DIAPLQEICC LASRYGALVF MDECHATGFL GPTGRGTDEL LGVMDQVTII NSTLGKALGG ASGGYTTGPG PLVSLLRQRA RPYLFSNSLP PAVVGCASKA LDLLMGSNTI VQSMAAKTQR FRSKMEAAGF TISGASHPIC PVMLGDARLA SRMADDMLKR GIFVIGFSYP VVPKGKARIR VQISAVHSEE DIDRCVEAFV EVGRLHGALP; Positive Samples: Mouse eye; Cellular Location: Mitochondrion, Nucleus

Target Information

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.